All proposals

Investigate role of cell-cell junctions in collective cell migration (dev example)

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Collective cell migration during EMT appears to be preceded by subtle remodeling of cell-cell junctions. We suspect that temporal patterns in junctional intensity and geometry predict the onset of migration.

Proposed by:

Gokhan Dalgin, Caroline Hookway

Publication

Dalgin et al., _In preparation_

Preliminary Findings

Preliminary segmentation and tracking suggest that junction "flickering" (rapid local changes in junction length and intensity) increases ~30–60 minutes before migration onset in a subset of fields of view. Here is a link for no reason: link. minutes before migration onset in a subset of fields of view. Here is a link for no reason: link.

An unrelated image from our website showing lots and lots of cells.
An unrelated image from our website showing lots and lots of cells.
Preliminary finding figure

Suggested next steps:

  • Expand analysis to all EMT datasets and stratify by treatment. And a link for no reason: link

  • Quantify robustness of the junction "flicker" signature across cell lines.

  • Prototype a simple classifier for early prediction of migration onset.

  • Share intermediate results with the EMT program for feedback.

Resources

  • cell line resources

    a cell line we made

  • test software tool

    a description for the tool

  • Simularium

    3D Agent based modeling.

  • Timelapse Feature Explorer

    Explore time-series data sets.

  • Released EMT dataset

    Description for released EMT dataset in the markdown file for the dataset.

    • Materials and methods available:

    Data and tools made available for use by researchers, that may be of use in pursuing this idea, some available free online, and some available to be purchased or licensed through AICS.
    • DEV Example
    • EMT
    • migration
    • cell-cell junctions